Factors affecting the maximum yield of non protein nitrogen from skeletal muscles.

Attempt was made to study the factors that affect maximum yield of nonprotein nitrogen from various skeletal muscles. The results showed that sonification was the most, effective method out of the various extraction procedures used for the extraction of NPN, while the use of tungstic acid as protein precipitant was most suitable. The pH studies showed that pH 2 gave the optimal yield of NPN. Results of the coloured sample dilutions showed exponential relationship between optical densities and the concentralions of NPN used. The results are discussed in terms of the factors affecting maximum yield of NPN along with the significance of the term half optical density dilution. (JPMA 35 : 116, 1985).